Letang is day-to-day with a lower-body injury, Penguins coach Mike Sullivan said. He did not participate in Pittsburgh's morning skate after playing 23:44 in a 4-0 loss to the Anaheim Ducks on Saturday.
Blue Jackets forwards Cam Atkinson and Alex Wennberg also will not play. Atkinson will miss 4-6 weeks with a broken foot sustained in a 2-1 win against the Philadelphia Flyers on Saturday. Wennberg, who injured his back in a 3-2 shootout loss at Pittsburgh on Thursday in which Letang scored the deciding goal, will also be out 4-6 weeks. Each was placed on injured reserve.
The Blue Jackets are 2-0-1 in their past three games, including the shootout loss at Pittsburgh six days ago that was one of two wins for the Penguins in their past seven games (2-5-0).