The Golden Knights were 4-2-1 without Fleury, who makes his first start since Nov. 23, a 4-2 home loss to the Edmonton Oilers.
Vegas forward Cody Glass will miss the first game of his NHL career after taking an elbow to the head from Rangers forward Brendan Lemieux on Sunday.
Goalie Corey Crawford is expected to start for the Blackhawks, who have points in three straight games (2-0-1) after going 1-5-1 in their previous seven.
The Blackhawks earned their first-ever win against the Golden Knights (1-5-2), a 5-3 victory in Las Vegas, on Nov. 13.