The two-time Vezina Trophy-winning goalie agreed to a seven-year contract with Florida on July 1.
Joel Quenneville, who won the Stanley Cup three times with the Chicago Blackhawks and is second in NHL history with 890 wins, will be coaching his first game with the Panthers.
The Lightning, who tied an NHL record with 62 wins last season, have won their past five season openers, the longest active streak in the NHL. This is the third straight year they have opened at home against the Panthers.
Lightning forward Nikita Kucherov led the NHL with 128 points (41 goals, 87 assists) last season, when he was voted winner of the Hart Trophy as the League's most valuable player.