The forward did not participate in the morning skate for the Bruins, who have won two straight, are 13-3-0 in their past 16 games and are seven points ahead of the Lightning for first place in the Atlantic and Eastern Conference.
If Marchand, Boston's second-leading scorer with 83 points (27 goals, 56 assists) is unable to play, forward Jake DeBrusk will take his place on the top line with Patrice Bergeron and David Pastrnak, who leads the Bruins in scoring with 91 points and has an NHL-best 47 goals.
The Lightning are 1-4-0 in their past five games following a Tampa Bay-record 11-game winning streak.