MacKinnon, fifth among NHL scorers with 93 points (35 goals, 58 assists) in 69 games, was injured in the second period of a 3-1 loss at the Los Angeles Kings on Monday.
The Avalanche, 8-2-2 in their past 12 games and 16-5-2 in their past 23, trail the St. Louis Blues by two points for the Central Division lead; the Blues play at the Anaheim Ducks on Wednesday.
Cale Makar will return for Colorado after missing five games with an upper-body injury sustained in a 3-2 win at the Nashville Predators on Feb. 29. The defenseman is second among NHL rookie scorers with 47 points (12 goals, 35 assists) in 56 games.
The Rangers, 2-4-0 in their past six games following a five-game winning streak, are three points behind the Carolina Hurricanes and Columbus Blue Jackets for the first wild card into the Stanley Cup Playoffs from the Eastern Conference.