Barkov, Florida's top-line center, missed the past three games because of a lower-body injury. He's second on the Panthers with 54 points (16 goals, 38 assists) in 50 games.
Florida, which is 1-2-1 following a season-long six-game winning streak and is coming off a 7-2 home loss to the Vegas Golden Knights on Thursday, has not lost consecutive games in regulation since losing three in a row Dec. 10-14.
Pittsburgh is 8-3-0 in its past 11 games after losing 4-2 at the Tampa Bay Lightning on Thursday. The Penguins have not lost consecutive games in regulation since Nov. 29-30.
The Panthers will be going for a season sweep of the Penguins after winning 4-2 at BB&T Center on Oct. 22 and 4-1 at PPG Paints Arena on Jan. 5.