Entering tonight's matchup, the Panthers (23-23-8) had been on a 6-2-0 run, with all six of those wins coming against teams currently holding a playoff spot, including an exciting 5-4 victory over the defending Stanley Cup champion Washington Capitals in overtime on Saturday night.
"We don't have much of a choice, obviously," said Panthers goaltender Roberto Luongo. "We've got a lot of important games coming up here. It's a disappointing result tonight for our club. We'll turn the page tomorrow and get ready for a big game on Tuesday [against the Dallas Stars]."
Playing in their third game in four nights, the Panthers struggled to find their footing in the first period against the Lightning, falling behind 1-0 after the first 20 minute. But in the second, the game really opened up, with both teams trading goals to make it a 3-2 game after two periods.
Florida finished the middle frame with a 17-3 advantage over Tampa in shots on goal.
"It was one of the best second periods we've had of the year," Panthers coach Bob Boughner said. "We've been saying it for a while: We've liked our composure no matter what happens. We've liked our compete. I thought we were the better team in the second period.
"You could tell in the beginning of the game that I thought we looked sluggish, but we found another level, which is important to see. It wasn't good enough tonight, but there's some good things this week. We've just got to sort of forget about today and come Tuesday ready to work."
After allowing three goals on 12 shots, Panthers goaltender James Reimer was relieved in net by Luongo to start the third period. Luongo went on to make nine saves as the Lightning potted a pair of power-play goals - including a 5-on-3 chance - in the final frame to secure the 5-2 win.
The Panthers finished the night with a 27-23 advantage in shots on goal.
"We have to [move on]," Panthers captain Aleksander Barkov said. "Of course, it happens. We don't want this to happen. We played against a really good team, back-to-back. We just need to forget this game and move forward."
Here are five takeaways from Sunday's loss in Sunrise…