The Senators cancelled practice Friday out of an abundance of caution with nine players in NHL COVID-19 protocol.
Matt Murray, Alex Formenton and Nikita Zaitsev were placed in protocol Thursday.
Murray, a goalie; Formenton, a forward; and Zaitsev, a defenseman, joined defenseman Josh Brown, Victor Mete and Nick Holden, and forwards Austin Watson, Connor Brown and Dylan Gambrell.
The Senators lost 2-0 to the Los Angeles Kings on Thursday.
"There's not a guy that I can say didn't give everything he had," Senators coach D.J. Smith said. "It's unfortunate when we're this shorthanded, but I thought our guys couldn't have played much harder."
Ottawa, which lost to the Boston Bruins 3-2 on Tuesday, did not practice Wednesday for precautionary reasons. The Senators also canceled practice Monday.
The Senators play the Pittsburgh Penguins on Saturday.
Forward Andrew Agozzino and defensemen Dillon Heatherington and Lassi Thompson were recalled from Belleville of the American Hockey League on Thursday.
Forwards Scott Sabourin and Egor Sokolov were recalled from Belleville on Monday, and defenseman Erik Brannstrom was recalled on Sunday. -- Calum Fraser