Each took contact during practice Friday for the first time since being injured, but were not in the lineup in a 5-1 loss to the St. Louis Blues on Saturday.
Letang, a 31-year-old defenseman, missed his 10th game since sustaining an upper-body injury during the 2019 Coors Light NHL Stadium Series against the Philadelphia Flyers on Feb. 23. Rust, a 26-year-old forward, missed his ninth game with a lower-body injury and has not played since Feb. 26.
"I feel pretty good," Letang said. "I've been skating forever, so the conditioning is there. That's for sure."
Pittsburgh (39-24-9) has won seven of 10 games (7-2-1) without Letang and six of nine (6-2-1) without Rust. It is third place in the Metropolitan Division, two points behind the New York Islanders and four behind the Washington Capitals. The Penguins are four points ahead of the fourth-place Carolina Hurricanes.
"There's a bunch of guys that have elevated their game, especially our leaders," Letang said. "You kind of see it in their game. Everybody kind of follows. We know we're in a difficult position. We have to fight for points."