The Syracuse Crunch have been the Lightning's American Hockey League (AHL) affiliate since the start of the 2012-13 season. The Crunch play in the AHL's Eastern Conference and home games are played at Oncenter War Memorial Arena.
The LHC Les Lions have been the Lightning's season partner since the 2014-15 season along with the Syracuse Crunch. The LHC Les Lions have called the Charlemagne Skating Rink home since 1967. The Lions returned to the Magnus League for the first time in 14 seasons in 2014.
The ECHL Solar Bears marked their first year as a Lightning affiliate in the 2018-19 season. The Solar Bears have been playing in Orlando since 2012 when they were added to the ECHL as an expansion team.