The center, who sustained a knee injury in a 4-0 loss against the Montreal Canadiens on Dec. 1, participated in the morning skate and coach Joel Quenneville said he was hopeful Barkov would return against the Pittsburgh Penguins on Saturday.
Florida, in third place in the Atlantic Division, is 7-1-1 in its past nine games but has been shut out twice in its past three games, including a 1-0 overtime loss at the Columbus Blue Jackets on Tuesday.
This will be the Panthers' first home game since Jan. 16.
The Golden Knights, in third place in the Pacific Division, will conclude a season-long eight-game road trip. They are 3-3-1 through the first seven games, including a 4-2 loss at the Tampa Bay Lightning on Tuesday. Overall, Vegas is 3-6-1 in its past 10 games after going 8-2-1 in its previous 11 games.
Golden Knights goalie Marc-Andre Fleury, who is expected to start, needs one victory to pass Henrik Lundqvist (459) for sole possession of fifth place on the NHL all-time list.