Stamkos missed the final 65 games last season after he tore the lateral meniscus in his right knee on Nov. 15, 2016.
"I'd say I was probably a little more nervous for the first exhibition game to be honest," Stamkos said. "That's kind of the first taste of the game in a long time. I think it's the normal anxiousness for the home opener and getting that first one under our belt."
Center Ryan Callahan, who missed 64 games last season because of two hip surgeries, will play for the Lightning. Defensemen Mikhail Sergachev, the No. 9 pick in the 2016 NHL draft, and Dan Girardi along with forward Chris Kunitz will make their debuts Tampa Bay.
Bob Boughner will make his debut as Panthers coach. Boughner, 46, who was an assistant with the San Jose Sharks each of the past two seasons, was hired on June 12.