The center did not play in a 5-2 win against the Maple Leafs in the first half of their home-and-home series at PPG Paints Arena on Tuesday because of an illness. Malkin failed to complete warmups and was scratched.
Malkin, who did not practice Wednesday, will travel with Pittsburgh to Toronto, Penguins coach Mike Sullivan said.
Malkin leads the Penguins with 58 points (18 goals, 40 assists) in 44 games but did not have a point in his past two games.
Rookie forward Anthony Angello entered the lineup Tuesday and scored his first NHL goal in his fifth NHL game.
Malkin missed 11 games in October with a lower-body injury and two games in December with an illness.
He tripped over Penguins defenseman Kris Letang and tumbled into the boards midway through the second period of a 7-2 win against the Blue Jackets on Oct. 5.
Malkin had 38 points (11 goals, 27 assists) in his 26 games when center Sidney Crosby was recovering from core muscle surgery. Pittsburgh was 18-6-4 in 28 games without Crosby (Nov. 12-Jan. 12).
The Penguins on Tuesday said forward Zach Aston-Reese is week to week with a lower-body injury. He was injured midway through the second period of a 5-1 win against the Detroit Red Wings on Sunday.
Aston-Reese had 13 points (six goals, seven assists) and was plus-6 in 57 games this season.
Pittsburgh also is without forwards Jake Guentzel (shoulder) and Nick Bjugstad (core). The Penguins on Feb. 10 traded for forward Jason Zucker.
