Coach Jon Cooper said it is not likely Bishop will be available Tuesday when the Lightning visit the Philadelphia Flyers.
"We play in less than 24 hours," he said, according to the Lightning website. "He pulled himself out tonight. It'd be hard to go in less than 24 hours."
Evgeni Nabokov replaced Bishop at the start of the second period.
Bishop allowed a shorthanded goal by Penguins forward Brandon Sutter with 51 seconds remaining in the first period. He made four saves on five shots before leaving the game, which was tied 1-1 at the end of the first. The Penguins scored three times against Nabokov on 21 shots to win the game 4-2.
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