The Ducks announced Souray's absence Tuesday.
The 37-year-old defenceman was hurt while working out off the ice on July 17, the team says. He had surgery July 25 in Los Angeles, and will be out at least four months from the time of the surgery.
Souray made a solid debut with the Ducks last fall, getting seven goals and 10 assists with a plus-19 rating as a key power-play contributor for the Pacific Division champions. Souray signed a three-year contract with Anaheim last summer.
Souray's lengthy absence is a blow to the Ducks, who also signed former Edmonton defenceman Mark Fistric.