Crosby played only two shifts in the third period of the Penguins' 4-1 loss to the Coyotes before leaving the ice. He did not return.
What happened to Crosby is unclear. Television replays showed Crosby skate to the Penguins bench, sit down with a grimace and try to collect himself.
Per team policy, the Penguins did not disclose details of Crosby's injury. Neither did Crosby.
"I don't even know what happened," he said after the game. "I had some discomfort. In the third period, I couldn't really do much."
Crosby finished with one shot on goal and was a minus-1 in 15:56 of ice time.
The Penguins listed him as day-to-day with an undisclosed injury. Pittsburgh's next game is at St. Louis on Saturday.