Coach Mike Babcock said Subban and Duchene will sit out Canada's opening game of the 2014 Sochi Olympics against Norway on Thursday (12 p.m. ET, USA, CBC), but will play Friday against Austria (12 p.m. ET, USA, CBC).
Tampa Bay Lightning right wing Martin St. Louis, who was a late addition to the team when Steven Stamkos was ruled out due to injury, and Vancouver Canucks defenseman Dan Hamhuis will play Thursday, with Hamhuis making his Olympic debut.
Babcock did not say whether St. Louis and Hamhuis will be replaced by Duchene and Subban, the reigning Norris Trophy winner, against Austria.
St. Louis and Hamhuis are each expected to play a role on Canada's special teams.
St. Louis was on the second power-play unit with Anaheim Ducks teammates Ryan Getzlaf and Corey Perry at practice Thursday, with Chicago Blackhawks teammates Duncan Keith and Patrick Sharp manning the points.
Here is the projected lineup for Canada against Norway: