The Penguins are down 13 players with sickness or injuries. They used a seventh defenseman in place of a fourth-line forward in a 4-3 shootout loss to the Florida Panthers on Monday.
However, the Penguins have managed to remain atop the Eastern Conference with 49 points (22-6-5). The Lightning have struggled to a 3-5-2 record in their past 10 games.
Tampa Bay is also winless in its past 10 regular-season games against Pittsburgh (0-9-1).
"They seem to be getting points regardless of who is in the lineup," Lightning coach Jon Cooper said. "And they have [Sidney Crosby] playing tonight, which they didn't have last time we played. [Evgeni] Malkin is playing. They are a dangerous team."