TIME: Wednesday, 7:30 p.m. EST.
The Senators are battling Boston and Toronto for the Northeast Division title and one of the top three seeds in the Eastern Conference. After facing Florida, Ottawa concludes its trip Friday at Philadelphia before finishing the regular season Saturday at home against Toronto.
Chris Phillips scored with one minute left in overtime, lifting the Senators to a wild 5-4 victory at Eastern Conference-leading Tampa Bay on Monday.
Captain Daniel Alfredsson, Marian Hossa and Mike Fisher scored for Ottawa, which allowed Dave Andreychuk's tying goal with 28 seconds left in regulation.
"I think we showed a lot of determination to come back and not give up, not to get down, continue to play hard even though this place was just rocking," Senators defenseman Brian Pothier said. "It's a fun time. It was like a playoff atmosphere."
Ottawa, 2-1-1 on the six-game trip, played without starting goalie Patrick Lalime, who sprained his left knee in Saturday's 2-2 tie against Toronto.
With Lalime not expected to play Wednesday, Martin Prusek will make his second consecutive start. Prusek is 2-0 with a .961 save percentage in two starts against the Panthers this season.
Alfredsson's 32nd goal was the 219th of his career, moving him past Alexei Yashin into first place on the Senators' career list.
Though the Panthers will miss the playoffs for the fourth straight year, they are assured of their best season since the 1999-00 team finished with 98 points.
Kristian Huselius and Matt Cullen scored power-play goals and Roberto Luongo stopped 28 shots as Florida ended a five-game losing streak with a 3-1 victory over Carolina on Monday.
"It's nice to win," Cullen said. "It's so much more fun to be around everyone when you win. These last three games are important for us. We want to get some wins."
The 28 saves was a relatively low total for Luongo, who has already faced 2,408 shots this season - the second-most in NHL history. Felix Potvin faced 2,438 with Toronto in 1996-97.
Florida has lost five straight home games against Ottawa.
STANDINGS: Senators - 98 points, 3rd place, 1 PB, Northeast Division. Panthers - 74 points, 4th place, 30 PB, Southeast Division.
TEAM LEADERS: Senators - Hossa, 35 goals; Alfredsson, 48 assists and 80 points; Chris Neil, 173 PIM. Panthers - Olli Jokinen, 24 goals, 28 assists and 52 points; Darcy Hordichuk, 158 PIM.
SPECIAL TEAMS (through March 29): Senators - Power play: 21.5 percent (78 for 362), 1st in NHL. Penalty killing: 83.4 percent (267 for 332), 20th. Panthers - Power play: 16.6 percent (49 for 296), 13th. Penalty killing: 86.4 percent (312 for 361), 2nd.
GOALTENDERS: Senators - Prusek (15-5-3, 3 SO, 1.99 GAA); Ray Emery (1-0-0, 1.00). Panthers - Luongo (25-32-13, 7, 2.34); Steve Shields (3-5-1, 3.38).
SEASON SERIES: Senators, 2-1.
LAST MEETING: Dec. 22; Senators, 3-2, OT. At Ottawa, Todd White scored 94 seconds into overtime, leading the Senators to their third straight win.
ROAD/HOME RECORDS: Senators - 18-15-5-1 on the road; Panthers - 16-14-6-3 at home.